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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
10/09/2013 |
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Autoria: |
TAN, B. H.; HALLORAN, G. M. |
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Título: |
Pollen dimorphism and the frequency of inductive anthers in anther culture of Triticum monococcum. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Biochemie und Physiologie der Pfanzen, v. 177, n. 2, p. 197-202, 1982. |
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Idioma: |
Inglês |
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Conteúdo: |
Summary
The frequenry of in vitro cultured Triticum monococcum anthers showing multicellular pollen development was with the exception of one instance significantly lower than the estimated frequency of anthers exhibiting pollen dimorphism at anthesis. A compamble frequency would support the hypothesis that multicellular pollens develop from the smaller form of dimorphic pollen. There was circumsumtial evidenre however in that the highest and lowest proportion of the smaller pollen in sampled dimorphic populations corresponded more or less to those of multicellular pollens observed in inductive anthers.
Interaction between culture medium and spike pretreatment precluded any generalization on their inductive efficacy.
One line of T. monococcum, designated no. 34, was used throughout the study. Plants were vernalized as seedlings for 4 weeks and raised in the glasshouse through autumn and winter in 1980; anthesis occurred 7 months after sowing.
The frequency of anthers showing pollen dimorphism at anthesis was estimated from a sample of 10 plants from which 2 spikes were randomJy sampled per plant and likewise 3 spikelets ( = 9 anthers) per spike. Pollen grains within individual anthers were removed on microscope slides and stained for at least 5 min with 1% acetocarmine. A minimum of 500 grains was scored at low magnification(s) for percent of small(er) non-staining pollen (see Tan and Halloran 1980) per anther sample.
For anther culture, spikes at the uninucleate stage, which corresponded to the awns emerging 1-2 cm from the flag leaf, were harvested with about 10 cm of the stem intact. These were subdivided into four treatment groups: (1) no pretreatment (control); (2) chilling at 12 °C dark for 5 d; (3) centrifugation of the spike at ca. 1,000 g for 10 min prior to anther inoculation; and (4) preatment (2) followed by (3).
The efficacy of three culture media were compared: (1) Nitsch and Nitsch's (1969) basal medium solidified with 0.9% agar and containing 6% sucrose, 0.75 mg/l each of NAA (naphthalene acetic acid) and 2,4-D (2,4-dichlorophenoxyacctic acid) plus 0.15 mg/l each of kinetin and zeatin, with the pH of the medium adjusted to 5.8 (medium designated NN); (2) NN medium as in (1) but incorporating in addition 30 ppm Ethrel (2-chlorethylphosphoric acid) (designated NN + E); and (3) the “New Potato Medium” (designated P2) as described by Chuang et al. (1978). Disposable 55 × 15 mm plastic petridishes were used as culture vessels. The media were sterilized by autoclaving at 1.05 kg/ cm2 for 15 min.
Chilled spike-tillers in groups of fives were stood in 50-ml Ehrlmeyer flasks containing either liquid basal NN or NN + E medium. Each group of spikes was hooded with a plastic bag to minimize desiccation. Those stood on basal NN medium were assigned to both NN and P2 media, whilst those on NN + E medium only to same but containing the phytohormones.
Before anthers were removed for inoculation, the awns were clipped and the whole spike was surface- sterilized with 70% ethanol. Cytological examination for m.c.p. development in cultured anthers was undertaken after 3-4 weeks' incubation at 25 °C/dark Anthers were removed and individually quashed in 1% acetocarmine. MenosSummary
The frequenry of in vitro cultured Triticum monococcum anthers showing multicellular pollen development was with the exception of one instance significantly lower than the estimated frequency of anthers exhibiting pollen dimorphism at anthesis. A compamble frequency would support the hypothesis that multicellular pollens develop from the smaller form of dimorphic pollen. There was circumsumtial evidenre however in that the highest and lowest proportion of the smaller pollen in sampled dimorphic populations corresponded more or less to those of multicellular pollens observed in inductive anthers.
Interaction between culture medium and spike pretreatment precluded any generalization on their inductive efficacy.
One line of T. monococcum, designated no. 34, was used throughout the study. Plants were vernalized as seedlings for 4 weeks and raised in the glasshouse through autumn and winter in 1980; anthesis occurred 7 months after sowing.
The frequency of anthers showing pollen dimorphism at anthesis was estimated from a sample of 10 plants from which 2 spikes were randomJy sampled per plant and likewise 3 spikelets ( = 9 anthers) per spike. Pollen grains within individual anthers were removed on microscope slides and stained for at least 5 min with 1% acetocarmine. A minimum of 500 grains was scored at low magnification(s) for percent of small(er) non-staining pollen (see Tan and Halloran 1980) per anther sample.
For anther culture, spikes at the uninucleate stage,... Mostrar Tudo |
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Palavras-Chave: |
Cultura de antera. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03692naa a2200145 a 4500
001 1835506
005 2013-09-10
008 1982 bl --- 0-- u #d
100 1 $aTAN, B. H.
245 $aPollen dimorphism and the frequency of inductive anthers in anther culture of Triticum monococcum.
260 $c1982
520 $aSummary The frequenry of in vitro cultured Triticum monococcum anthers showing multicellular pollen development was with the exception of one instance significantly lower than the estimated frequency of anthers exhibiting pollen dimorphism at anthesis. A compamble frequency would support the hypothesis that multicellular pollens develop from the smaller form of dimorphic pollen. There was circumsumtial evidenre however in that the highest and lowest proportion of the smaller pollen in sampled dimorphic populations corresponded more or less to those of multicellular pollens observed in inductive anthers. Interaction between culture medium and spike pretreatment precluded any generalization on their inductive efficacy. One line of T. monococcum, designated no. 34, was used throughout the study. Plants were vernalized as seedlings for 4 weeks and raised in the glasshouse through autumn and winter in 1980; anthesis occurred 7 months after sowing. The frequency of anthers showing pollen dimorphism at anthesis was estimated from a sample of 10 plants from which 2 spikes were randomJy sampled per plant and likewise 3 spikelets ( = 9 anthers) per spike. Pollen grains within individual anthers were removed on microscope slides and stained for at least 5 min with 1% acetocarmine. A minimum of 500 grains was scored at low magnification(s) for percent of small(er) non-staining pollen (see Tan and Halloran 1980) per anther sample. For anther culture, spikes at the uninucleate stage, which corresponded to the awns emerging 1-2 cm from the flag leaf, were harvested with about 10 cm of the stem intact. These were subdivided into four treatment groups: (1) no pretreatment (control); (2) chilling at 12 °C dark for 5 d; (3) centrifugation of the spike at ca. 1,000 g for 10 min prior to anther inoculation; and (4) preatment (2) followed by (3). The efficacy of three culture media were compared: (1) Nitsch and Nitsch's (1969) basal medium solidified with 0.9% agar and containing 6% sucrose, 0.75 mg/l each of NAA (naphthalene acetic acid) and 2,4-D (2,4-dichlorophenoxyacctic acid) plus 0.15 mg/l each of kinetin and zeatin, with the pH of the medium adjusted to 5.8 (medium designated NN); (2) NN medium as in (1) but incorporating in addition 30 ppm Ethrel (2-chlorethylphosphoric acid) (designated NN + E); and (3) the “New Potato Medium” (designated P2) as described by Chuang et al. (1978). Disposable 55 × 15 mm plastic petridishes were used as culture vessels. The media were sterilized by autoclaving at 1.05 kg/ cm2 for 15 min. Chilled spike-tillers in groups of fives were stood in 50-ml Ehrlmeyer flasks containing either liquid basal NN or NN + E medium. Each group of spikes was hooded with a plastic bag to minimize desiccation. Those stood on basal NN medium were assigned to both NN and P2 media, whilst those on NN + E medium only to same but containing the phytohormones. Before anthers were removed for inoculation, the awns were clipped and the whole spike was surface- sterilized with 70% ethanol. Cytological examination for m.c.p. development in cultured anthers was undertaken after 3-4 weeks' incubation at 25 °C/dark Anthers were removed and individually quashed in 1% acetocarmine.
653 $aCultura de antera
700 1 $aHALLORAN, G. M.
773 $tBiochemie und Physiologie der Pfanzen$gv. 177, n. 2, p. 197-202, 1982.
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Embrapa Trigo (CNPT) |
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| 2. |  | TAN, B.; GRATTAPAGLIA, D.; MARTINS, G. S.; FERREIRA, K. Z.; SUNDBERG, B.; INGVARSSON, P. K. Evaluating the accuracy of genomic prediction of growth and wood traits in two Eucalyptus species and their F1 hybrids. BMC plant biology, v. 17, n. 1, p. 110, 2017. (Open Access).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| 3. | | GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da; RESENDE, R. T.; CAPPA, E. P.; MÜLLER, B. S. F.; TAN, B.; ISIK, F.; RATCLIFFE, B.; EL-KASSABY, Y. A. Quantitative genetics and genomics converge to accelerate forest tree breeding. Frontiers in Plant Science, v. 9, article 1693, 2018. Na publicação: Orzenil B. Silva-Junior.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| 4. | | GRATTAPAGLIA, D.; RESENDE, R.; MÜLLER, B.; LIMA, B.; TAN, B.; TAKAHASHI, E.; ESTOPA, R.; GARCIA, C.; ROSSE, L.; SILVA JUNIOR, O. Genomic prediction of complex traits: genomics and quantitative genetics converge to innovate Eucalyptus breeding in Brazil. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 174. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
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